| 1965 | Czechoslovak Grand Prix · Grand Prix | Mike Hailwood | MV Agusta | — |
| 1966 | Czechoslovak Grand Prix · Grand Prix | Mike Hailwood | Honda | — |
| 1967 | Czechoslovak Grand Prix · Grand Prix | Mike Hailwood | Honda | — |
| 1968 | Czechoslovak Grand Prix · Grand Prix | Giacomo Agostini | MV Agusta | — |
| 1969 | Czechoslovak Grand Prix · Grand Prix | Giacomo Agostini | MV Agusta | — |
| 1972 | Czechoslovak Grand Prix · Grand Prix | Giacomo Agostini | MV Agusta | — |
| 1973 | Czechoslovak Grand Prix · Grand Prix | Giacomo Agostini | MV Agusta | — |
| 1974 | Czechoslovak Grand Prix · Grand Prix | Phil Read | MV Agusta | — |
| 1975 | Czechoslovak Grand Prix · Grand Prix | Phil Read | MV Agusta | — |
| 1976 | Czechoslovak Grand Prix · Grand Prix | John Newbold | Suzuki | — |
| 1977 | Czechoslovak Grand Prix · Grand Prix | Johnny Cecotto | Yamaha | — |
| 1987 | Czechoslovak Grand Prix · Grand Prix | Wayne Gardner | Honda | Wayne Gardner |
| 1988 | Czechoslovak Grand Prix · Grand Prix | Wayne Gardner | Honda | — |
| 1989 | Czechoslovak Grand Prix · Grand Prix | Kevin Schwantz | Suzuki | — |
| 1990 | Czechoslovak Grand Prix · Grand Prix | Wayne Rainey | Yamaha | Kevin Schwantz |
| 1991 | Czechoslovak Grand Prix · Grand Prix | Wayne Rainey | Yamaha | Wayne Rainey |
| 1993 | Czech Republic motorcycle Grand Prix · Grand Prix | Wayne Rainey | Yamaha | Wayne Rainey |
| 1994 | Czech Republic motorcycle Grand Prix · Grand Prix | Mick Doohan | Honda | Luca Cadalora |
| 1995 | Czech Republic motorcycle Grand Prix · Grand Prix | Luca Cadalora | Yamaha | Luca Cadalora |
| 1996 | Czech Republic motorcycle Grand Prix · Grand Prix | Àlex Crivillé | Honda | Jean-Michel Bayle |
| 1997 | Czech Republic motorcycle Grand Prix · Grand Prix | Mick Doohan | Honda | Mick Doohan |
| 1998 | Czech Republic motorcycle Grand Prix · Grand Prix | Max Biaggi | Honda | Mick Doohan |
| 1999 | Czech Republic motorcycle Grand Prix · Grand Prix | Tadayuki Okada | Honda | Jurgen van den Goorbergh |
| 2000 | Czech Republic motorcycle Grand Prix · Grand Prix | Max Biaggi | Yamaha | Max Biaggi |
| 2001 | Czech Republic motorcycle Grand Prix · Grand Prix | Valentino Rossi | Honda | Max Biaggi |
| 2002 | Gauloises Grand Prix České republiky · Grand Prix | Max Biaggi | Yamaha | Max Biaggi |
| 2003 | Gauloises Grand Prix České republiky · Grand Prix | Valentino Rossi | Honda | Valentino Rossi |
| 2004 | Gauloises Grand Prix České republiky · Grand Prix | Sete Gibernau | Honda | Sete Gibernau |
| 2005 | Gauloises Grand Prix České republiky · Grand Prix | Valentino Rossi | Yamaha | Sete Gibernau |
| 2006 | Gauloises Grand Prix České republiky · Grand Prix | Loris Capirossi | Ducati | Valentino Rossi |
| 2007 | Cardion AB Grand Prix České republiky · Grand Prix | Casey Stoner | Ducati | Casey Stoner |
| 2008 | Cardion ab Grand Prix České republiky · Grand Prix | Valentino Rossi | Yamaha | Casey Stoner |
| 2009 | Cardion ab Grand Prix České republiky · Grand Prix | Valentino Rossi | Yamaha | Valentino Rossi |
| 2010 | Cardion ab Grand Prix České republiky · Grand Prix | Jorge Lorenzo | Yamaha | Dani Pedrosa |
| 2011 | Cardion AB Grand Prix České republiky · Grand Prix | Casey Stoner | Honda | Dani Pedrosa |
| 2012 | bwin Grand Prix České republiky · Grand Prix | Dani Pedrosa | Honda | Jorge Lorenzo |
| 2013 | bwin Grand Prix České republiky · Grand Prix | Marc Márquez | Honda | Cal Crutchlow |
| 2014 | bwin Grand Prix České republiky · Grand Prix | Dani Pedrosa | Honda | Marc Márquez |
| 2015 | bwin Grand Prix České republiky · Grand Prix | Jorge Lorenzo | Yamaha | Jorge Lorenzo |
| 2016 | HJC Helmets Grand Prix České republiky · Grand Prix | Cal Crutchlow | Honda | Marc Márquez |
| 2017 | Monster Energy Grand Prix České republiky · Grand Prix | Marc Márquez | Honda | Marc Márquez |
| 2018 | Monster Energy Grand Prix České republiky · Grand Prix | Andrea Dovizioso | Ducati | Andrea Dovizioso |
| 2019 | Monster Energy Grand Prix České republiky · Grand Prix | Marc Márquez | Honda | Marc Márquez |
| 2020 | Monster Energy Grand Prix České republiky · Grand Prix | Brad Binder | KTM | Johann Zarco |
| 2025 | Tissot Grand Prix of Czechia · Grand Prix | Marc Márquez | Ducati | Francesco Bagnaia |
| 2025 | Tissot Grand Prix of Czechia · Sprint | Marc Márquez | Ducati | — |
| 2026 | Monster Energy Grand Prix of Czechia · Grand Prix | Marc Márquez | Ducati | Ai Ogura |
| 2026 | Monster Energy Grand Prix of Czechia · Sprint | Francesco Bagnaia | Ducati | — |